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Infectious Disease >> H.Pylori IgG

  • Productname : 

    Helicobacter pylori IgG
  • Catalognumber : 

  • Productspecification : 

  • Productpurpose : 

    Helicobacter pylori IgG Chemiluminescence ELISA is intended for use in evaluating the serologic status to H. pylori infection in patients with gastrointestinal symptoms.
  • Productprinciple : 

    Purified H. pylori antigen is coated on the surface of microwells.Diluted patient serum is added to wells, and the H. pylori IgG specific antibody, if present, binds to the antigen. All unbound materials are washed away. After adding enzyme conjugate, it binds to the antibody-antigen complex. Excess enzyme conjugate is washed off and substrate A & substrate B mixture is added. The light generated (RLU) is proportional to the amount of IgG specific antibody in the sample.The results are read by a microwell luminometer compared in a parallel manner with calibrator and controls.

  • Maincomposition : 

    1.Microwell Strips: purified H. pylori antigen coated wells (12 x 8 wells)

    2.Sample Diluent: Blue color solution. 1 vial (22 mL)

    3.Calibrator: Factor value ( f ) stated on label. Red Cap.1 vial (150 µL)

    4.Negative Control: Range stated on label. Natural Cap.1 vial (150 µL)

    5.Positive Control: Range stated on label. Green Cap. 1 vial (150 µL)

    6.Washing Concentrate 10x. 1 bottle (100 mL)

    7.Enzyme Conjugate: Red color solution. 1 vial (12 mL)

    8.Substrate A: H2O2 in buffer. Natural bottle. 1 vial (6 mL)

    9.Substrate B: Luminol in buffer. Amber bottle. 1 vial (6 mL)