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Steroid Tests >> Estradiol(E2)

  • Productname : 

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  • Productpurpose : 

    Enzyme Immunoassay for the Quantitative Determination of ESTRADIOL (E2) In Human Serum or plasma
  • Productprinciple : 

    The E2 EIA is based on the principle of competitive binding between E2 in the test specimen and E2-HRP conjugate for a constant amount of rabbit anti-Estradiol. In the incubation, goat anti-rabbit IgG-coated wells are incubated with 25µL E2 standards, controls,patient samples, 100 µL Estradiol-HRP Conjugate Reagent and 50µL rabbit anti-Estradiol reagent at room temperature (18-25°C) for 90 minutes. During the incubation, a fixed amount of HRP-labeled E2 competes with the endogenous E2 in the standard, sample, or quality control serum for a fixed number of binding sites of the specific E2 antibody. Thus, the amount of E2 peroxidase conjugate immunologically bound to the well progressively decreases as the concentration of E2 in the specimen increases.

    Unbound E2 peroxidase conjugate is then removed and the wells washed. Next, a solution of TMB Reagent is added and incubated at room temperature for 20 minutes, resulting in the development of blue color. The color development is stopped with the addition of stop solution, and the absorbance is measured spectrophotometrically at 450 nm. The intensity of the color formed is proportional to the amount of enzyme present and is inversely related to the amount of unlabeled E2 in the sample. A standard curve is obtained by plotting the concentration of the standard versus the absorbance.The E2 concentration of the specimens and controls run concurrently with the standards can be calculated from the standard curve.

  • Maincomposition : 

    Goat Anti-Rabbit IgG-coated microtiter wells, 96 wells

    Estradiol Reference Standards: 0, 10, 30,100, 300 and 1000 Pg/mL

    Liquid, 0.50 mL each, ready to use.

    Rabbit Anti-Estradiol Reagent , 7.0 mL

    Estradiol-HRP Conjugate Reagent , 12 mL

    Wash Buffer Concentrate (50X),15 mL

    TMB Substrate , 12 mL.

    Stop Solution , 12 mL.